normal human urothelial sv huc 1 cells  (ATCC)

Journal: PLOS One

Article Title: Integrated light and electron microscopy workflow for morphological, molecular and ultrastructural analysis of spheroids

doi: 10.1371/journal.pone.0342659

Figure Lengend Snippet: Representative haematoxylin–eosin (HE)-stained sections of SV-HUC-1 and T24 spheroids prepared by cryosectioning ( A-F ) and paraffin embedding (G-L) . A necrotic core (indicated by asterisks, A-B , D-E , G-H , J-K ) is visible in most cross sections, except those obtained from peripheral regions. Scale bars: 500 µm (A, D, G, J), 100 µm (B, E, H, K), 50 µm (C, F, I, L).

Article Snippet: Normal human urothelial SV-HUC-1 cells (CRL-9520) and muscle-invasive human bladder cancer urothelial T24 cells (HTB-4) were purchased from ATCC (Manassas, VA, United States) and cultured in a 1:1 mixture of A-DMEM medium (Gibco, Thermo Fisher Scientific, Waltham, MA, United States) and F12 (Sigma-Aldrich, St. Louis, MO, United States), supplemented with 5% fetal bovine serum (Invitrogen, Carlsbad, CA, United States) and 4 mM GlutaMAX (Gibco, Thermo Fisher Scientific, Waltham, MA, United States).

Techniques: Staining

Journal: PLOS One

Article Title: Integrated light and electron microscopy workflow for morphological, molecular and ultrastructural analysis of spheroids

doi: 10.1371/journal.pone.0342659

Figure Lengend Snippet: Representative images of paraffin sections show E-cadherin (green) in the plasma membrane in cells of SV-HUC-1 spheroids ( A-a3 ) and N-cadherin (red) in the plasma membrane of T24 spheroids ( B-b3 ). Some SV-HUC-1 cells are also positive for N-cadherin (arrows, a2 and a3 ). Yellow insets (in A and B) are magnified (a1-a3 and b1-b3) and display individual and merged channels of E-cadherin, N-cadherin, and DAPI-stained nuclei. Scale bars: 100 µm (A, B) , 20 µm (a1-a3, b1-b3).

Article Snippet: Normal human urothelial SV-HUC-1 cells (CRL-9520) and muscle-invasive human bladder cancer urothelial T24 cells (HTB-4) were purchased from ATCC (Manassas, VA, United States) and cultured in a 1:1 mixture of A-DMEM medium (Gibco, Thermo Fisher Scientific, Waltham, MA, United States) and F12 (Sigma-Aldrich, St. Louis, MO, United States), supplemented with 5% fetal bovine serum (Invitrogen, Carlsbad, CA, United States) and 4 mM GlutaMAX (Gibco, Thermo Fisher Scientific, Waltham, MA, United States).

Techniques: Clinical Proteomics, Membrane, Staining

Journal: PLOS One

Article Title: Integrated light and electron microscopy workflow for morphological, molecular and ultrastructural analysis of spheroids

doi: 10.1371/journal.pone.0342659

Figure Lengend Snippet: SV-HUC-1 (A) and T24 cells (C) form spheroids with a spherical morphology. (B) SV-HUC-1 cells are tightly attached to each other (arrows), and microvilli are seen on their surface. (D) T24 cells are loosely attached, displaying wider intercellular spaces (arrowheads) and have fewer microvilli. Scale bars: 100 µm (A, C) , 10 µm (B, D) .

Article Snippet: Normal human urothelial SV-HUC-1 cells (CRL-9520) and muscle-invasive human bladder cancer urothelial T24 cells (HTB-4) were purchased from ATCC (Manassas, VA, United States) and cultured in a 1:1 mixture of A-DMEM medium (Gibco, Thermo Fisher Scientific, Waltham, MA, United States) and F12 (Sigma-Aldrich, St. Louis, MO, United States), supplemented with 5% fetal bovine serum (Invitrogen, Carlsbad, CA, United States) and 4 mM GlutaMAX (Gibco, Thermo Fisher Scientific, Waltham, MA, United States).

Techniques:

Journal: PLOS One

Article Title: Integrated light and electron microscopy workflow for morphological, molecular and ultrastructural analysis of spheroids

doi: 10.1371/journal.pone.0342659

Figure Lengend Snippet: Outermost cells in SV-HUC-1 spheroids display cuboidal morphology (A) , whereas T24 cells are more elongated (B) . The presence of cell junctions in the outermost cell layer of SV-HUC-1 (C) and T24 spheroid (D) (boxed regions) indicates a tight cellular network of the outermost cell layer. The central necrotic zone (asterisks) is filled with necrotic cells in SV-HUC-1 and T24 spheroids (E, F) , as clearly demonstrated on semithin sections (G, H) , prepared before ultrathin sectioning. Scale bars: 100 µm (G-H) , 10 µm (A-B) , 5 µm (E-F) , 1 µm (C-D) .

Article Snippet: Normal human urothelial SV-HUC-1 cells (CRL-9520) and muscle-invasive human bladder cancer urothelial T24 cells (HTB-4) were purchased from ATCC (Manassas, VA, United States) and cultured in a 1:1 mixture of A-DMEM medium (Gibco, Thermo Fisher Scientific, Waltham, MA, United States) and F12 (Sigma-Aldrich, St. Louis, MO, United States), supplemented with 5% fetal bovine serum (Invitrogen, Carlsbad, CA, United States) and 4 mM GlutaMAX (Gibco, Thermo Fisher Scientific, Waltham, MA, United States).

Techniques:

Journal: Journal of experimental & clinical cancer research : CR

Article Title: High and selective cytotoxicity of ex vivo expanded allogeneic human natural killer cells from peripheral blood against bladder cancer: implications for natural killer cell instillation after transurethral resection of bladder tumor.

doi: 10.1186/s13046-024-02955-7

Figure Lengend Snippet: Fig. 2 Comparison of cytotoxicity of chemotherapeutic agents against human BCa cell lines and normal urothelial cells in vitro. (A–D) Comparison of the killing rates of mitomycin at different concentration (0.5 and 1.0 mg/mL) against human BCa cell lines including BIU-87 (A), T24 (B), UMUC-3 (C), 5637 (D), and normal urothelial cell SV-HUC-1 measured using the CCK-8 assay at 0.5, 1, and 2 h (n = 3); E–H Comparison of killing rate of epirubicin at differ ent concentration (0.5, 1.0 mg/mL) against human BCa cell lines including BIU-87 (E), T24 (F), UMUC-3 (G), 5637 (H), and normal urothelial cell SV-HUC-1 measured using the CCK-8 assay at 0.5, 1, and 2 h (n = 3). Data expressed as means ± SD were plotted, and Student’s t-test was used to compare two independent groups (A–H). *p < 0.05; ns, not significant

Article Snippet: Human BCa cell lines (T24, UMUC-3, and 5637) and normal human urothelial cell line (SV-HUC-1) were obtained from the American Type Culture Collection.

Techniques: Comparison, In Vitro, Concentration Assay, CCK-8 Assay

Journal: Journal of experimental & clinical cancer research : CR

Article Title: High and selective cytotoxicity of ex vivo expanded allogeneic human natural killer cells from peripheral blood against bladder cancer: implications for natural killer cell instillation after transurethral resection of bladder tumor.

doi: 10.1186/s13046-024-02955-7

Figure Lengend Snippet: Fig. 3 Expression of MHC-I, MICA/B, ULBP-2/5/6, and B7-H6 on surfaces of BCa and urothelial cells. (A) MHC-I expression on human BCa cell lines, includ ing BIU-87, T24, UMUC-3, 5637, and normal urothelial cell line SV-HUC-1, were detected using flow cytometry. Representative images and summary data of the MFI for MHC-I are shown (n = 3); (B) MICA/B expression on human BCa cell lines and normal urothelial cell line SV-HUC-1 were detected using flow cytometry. Representative images and summary data of the percentages of MICA/B + cells are shown (n = 3); (C) ULBP-2/5/6 expressions on human BCa cell lines and normal urothelial cell line SV-HUC-1 were detected using flow cytometry. Representative images and summary data of the percentages of ULBP-2/5/6 + cells are shown (n = 3); (D) B7-H6 expression on human BCa cell lines and normal urothelial cell line SV-HUC-1 was detected using flow cytometry. Representative images and summary data of the percentages of B7-H6 + cells are shown (n = 3). All bars represent the means ± SD. Statistical significance was determined using an unpaired t-test (A-D). *p < 0.05. Abbreviations: MHC-I: major histocompatibility complex class I, MICA/B: MHC-I polypeptide-related sequences A and B, ULBP-2/5/6: cytomegalovirus UL16-binding protein-2/5/6; BCa: bladder cancer

Article Snippet: Human BCa cell lines (T24, UMUC-3, and 5637) and normal human urothelial cell line (SV-HUC-1) were obtained from the American Type Culture Collection.

Techniques: Expressing, Flow Cytometry, Immunopeptidomics, Binding Assay